M. Saqib Shahzad, S. Yassir Abbas Bokhari, Abdul Qayyum Rao, M. Hashim Raza, Obaid Ullah, Zia-ur-Rahman, A. Ali Shahid, Zahoor Ahmad, S. Riazuddin

Population Studies for STR Loci (D3S1358, D5S818, D7S820, D18S51 and FGA) in NWFP and Sindhi Populations of Pakistan for Forensic Use

Badania populacyjne loci STR (D3S1358, D5S818, D7S820, D18S51 i FGA) w wybranych populacjach

Pakistanu (NWFP i Sindhi) w praktyce sądowo-lekarskiej

Centre of Excellence in Molecular Biology (CEMB), University of the Punjab, Lahore, Pakistan.

CEMB’s Forensic DNA typing project is directed towards the introduction of DNA typing technique in Pakistan’s criminal justice system so as to exonerate an innocent and wrongly accused person and incriminates the culprit. The present study is a part of the project of CEMB to analyze Sindhi and NWFP (North West Frontier Province) populations for five STR (Short Tandem Repeat) loci out of 13 CODIS (Combined DNA

Index System) loci. Allelic frequencies and heterozygosity for STR markers D3S1358, D5S818, D7S820, D18S51 and FGA (FIBRA) were determined. Samples from unrelated individuals were amplified by multiplex PCR using the unlabelled primers for these markers followed by denaturing Polyacrylamide Gel Electrophoresis (PAGE).